- Design and implementation of epidemiological surveys
- Design of field studies
- Measurement of food intake (frequency surveys, prospective surveys, food intake patterns)
- Physical activity and other habits assessment
- Prospective collections of Biobank-stored biological samples (serum, plasma, whole blood)
- Genotyping
- Epigenetic (methylation patterns and miRNAs determination)
- Assessment of biomarkers in blood and urine samples
- Databases implementation
- Statistical analysis of data, automated learning
- Bioinformatic analysis
- SNP Genotyping
- Immunoassays
Clinical Research
- Surveys for screening of psychological alterations associated with diabetes
- Psychometric analysis
- Cost-effectiveness analysis of ICTs applied to diabetes
- ICTs applied to health: Application of a new online tool for the treatment of
depression in patients with type 1 diabetes https://biopsicosocialdiabetes.com/ - Clinical studies in people with T1D
- Telemedicine in T1D
- Implementation and monitorization of new diabetes technologies
- Analysis of amino acids and acylcarnitines in dried blood by MS / MS
- Simultaneous determination in dried blood of methylmalonic acid, methyl citric
acid and total homocysteine by LC-MS / MS. - Determination of immunoreactive trypsinogen and thyrotropin in dried blood by
immunofluorimetric method (DELFIA).
- Determination of hemoglobin variants in dried blood by HPLC
- Cuantification of chloride in sweat (diagnosis of cystic fibrosis).
- Body composition techniques (Bioelectrical Impedance, Applied anthropometry)
- Hand grip dynamometry and legs
- Indirect calorimetry
- Dual-Energy X-ray Absorptiometry
- Muscle ultrasound
- Software for assessment of muscle composition at the level of L3 on CT
- Assessment of dietary intake (Frequency and prospective surveys, Food intake patterns, Own software for intake assessment: Dietstat®)
- Screening tools and structured nutritional assessment (Subjective global assessment, MNA®, MUST, NRS 2002, SNAQ)
- Exercise valuation (6 minute walk test SPPB, Physical activity questionnaires (IPAQ), Cardiopulmonary exercise test, Accelerometers (wGT3X ActiGraph®))
- Dysphagia screening and assessment tools (Volume-viscosity test (MECV-V),
EAT 10 ®, Water test) - Quality of life assessment (SF 12, QOLB Spain, CFQR Spain, St George)
- Assessment of psychological symptoms (anxiety and depression, HADS)
- Participation in national and international registries for bronchiectasis,inpatients and outpatients artificial nutrition (NADYA), cystic fibrosis, outpatients parenteral nutrition
- Prospective collections of samples (serum, plasma, whole blood, feces) from cystic fibrosis, bronchiectasis, parenteral nutrition and diabetes, obesity, type 1 diabetes, type 2 diabetes, chronic kidney disease)
- Observational Clinical Trials
- Interventional Clinical Trials, Phases I-IV
- Nutritional Intervention Clinical trials
Basic and translational research:
- Quantification of gene expression in PBMCs or tissue by RT qPCR
- Protein expression by Immunohistochemistry, immunocytochemistry, immunofluorescence, electron microscopy and Western Blot
- Analysis of DNA-protein interaction by EMSA assay
- Analysis of epigenetic changes (miRNAs and DNA methylation)
- Bioinformatic analysis of miRNAs (function, target genes) by Mirtabase,DAVID, PANTHER, Cytoscape software
- Quantification of hormones and cytokines by ELISA and multiplex assays in plasma/serum and cell/tissue extracts
- Isolation, phenotyping, culture and differentiation of mesenchimal stem cells derived from adipose tissue to white, beige and brown adipocytes, osteocytes and endothelial cells
- Tissue culture of adipose and brain, cell lines and primary culture of human, mouse and rat adipocytes, peripheral cells and macrophages
- Human and murine islets of Langerhans handling, including fresh murine islet isolation and ex vivo study of insulin and glucagon secretion, viability, apoptosis and proliferation
- In vitro experiments in beta cell lines (MIN-6)
- Mouse models for the study of the cannabinoid type 1 receptor (CB1R): Global CB1R knockout mice and inducible beta cell-specific CB1R knockout mice (MIP-Cre CNR1 LoxP/LoxP )
- Type 1 diabetes mouse models: Non-Obese Diabetic (NOD) and streptozotocin (STZ)-injected mice
- Mouse models of high-fat-diet-induced obesity, prediabetes and type 2 diabetes
- Metabolic assessment of the effects of drugs and food supplements in animal models of obesity, pre-diabetes and type 2 diabetes
- Analysis of inflammatory and oxidative processes in animal models
